田 合，王苗苗，严 欢，李慕春^*

（新疆维吾尔自治区分析测试研究院，乌鲁木齐 830011）

摘要：蜡菊具有显著的抗氧化活性，黄酮是其主要的抗氧化成分，为充分利用蜡菊资源，该研究采用响应面实验确定了蜡菊总黄酮的最佳提取工艺，并对蜡菊抗氧化活性进行系统评价。结果表明，蜡菊总黄酮的最佳提取工艺为：乙醇体积分数53%、提取温度70 ℃、提取时间1.0 h、料液比1:50 g/mL。在此工艺条件下，蜡菊总黄酮含量为 (338.43±0.71) mg/g。抗氧化活性评价结果表明，蜡菊总黄酮对DPPH和ABTS＋自由基的清除效果显著，在实验考察范围内，总黄酮的浓度与其抗氧化能力呈显著的正相关 (P＜0.05) ，有较强的抗氧化能力。该研究不仅为蜡菊中总黄酮的高效提取提供了可靠的工艺参数，也为蜡菊资源的综合开发利用提供参考依据。

关键词：蜡菊；总黄酮；响应面法；抗氧化活性

中图分类号：R284.2 文献标识码：A 文章编号：1674-506X（2024）04-0008-0007

Optimization of Total Flavonoid Extraction from Helichrysum ledifolia and Assessment of Its Antioxidant Activity in Vitro

TIAN He，WANG Miaomiao，YAN Huan，LI Muchun^*

（Xinjiang Academic Institute of Analysis and Testing，Urumqi 830011，China）

Abstract：Helichrysum has significant antioxidant activity，and flavonoids are its main antioxidant components. In order to make full use of its resources， the optimal extraction process of total flavonoids was determined by response surface experiment in this study， and the antioxidant activity of Helichrysum was systematically evaluated. The results showed that the optimal extraction technology of total flavonoids was as follows：ethanol volume fraction 53%，extraction temperature 70 ℃，extraction time 1.0 h，solid-liquid ratio 1:50g/mL. Under this process condition， the total flavonoid content was (338.43 ± 0.71) mg/g. The results of antioxidant activity evaluation showed that total flavonoids had a significant scavenging effect on DPPH and ABTS＋ free radicals，and the concentration of total flavonoids had a significant positive correlation with its antioxidant capacity (P＜0.05) ， indicating a strong antioxidant capacity. This study not only provides reliable technological parameters for the efficient extraction of total flavonoids from Helichrysum，but also provides a reference for the comprehensive development and utilization of Helichrysum resources.

Keywords：Helichrysum ledifolia；total flavonoids；response surface method；antioxidant activity

doi：10.3969/j.issn.1674-506X.2024.04-002

收稿日期：2023-07-21

作者简介：田合（1998-），女，本科，研究实习员。研究方向：天然产物。

*通信作者：李慕春（1978-），男，硕士，研究员。研究方向：天然产物及绿色化学。

引用格式：田合，王苗苗，严欢，等. 蜡菊总黄酮提取工艺优化及体外抗氧化活性评价［J］. 食品与发酵科技，2024，60（4）：8-14.